I have a plan to inoculate some (pre-serile) jars, and wanted to put out my plan to make sure I'm not forgetting something.
I've read a lot of stuff (PF Tek, videos, etc), but still have a few questions.
I plan to line a cabinet with plastic liner to make sure there's nothing on the walls.
Spray a lot of lysol into the cabinet and shut it a few times to try to kill everything in there.
Question: Can I use 70% Isopropyl alcohol to clean off gloves, etc, or do I need to go out and find some higher percentage?
Question: I got some "professional lysol disinfectant spray" (unfortunately with linen scent). Is this appropriate?
Then I'll start with the jars, take off foil, inoculate through holes as quickly as possible.
After inoculating, I plan to put them in a box and store them in the cabinet. (probably about 70 degrees or more when the spring comes).
Question: After the needle is out, do I have to keep jars in a sterile environment, or am I pretty safe? Should I spray lysol into the cabinet every so often, or could that damage the stuff I want to grow?
Question: Do they need air to grow? There will be some (obviously), but should I open cabinet and box periodically to get some fresh air in, or would that be bad?
I know there may not be definite answers, but if anything is a "red flag" please let me know.
Thank you so much.
I've read a lot of stuff (PF Tek, videos, etc), but still have a few questions.
I plan to line a cabinet with plastic liner to make sure there's nothing on the walls.
Spray a lot of lysol into the cabinet and shut it a few times to try to kill everything in there.
Question: Can I use 70% Isopropyl alcohol to clean off gloves, etc, or do I need to go out and find some higher percentage?
Question: I got some "professional lysol disinfectant spray" (unfortunately with linen scent). Is this appropriate?
Then I'll start with the jars, take off foil, inoculate through holes as quickly as possible.
After inoculating, I plan to put them in a box and store them in the cabinet. (probably about 70 degrees or more when the spring comes).
Question: After the needle is out, do I have to keep jars in a sterile environment, or am I pretty safe? Should I spray lysol into the cabinet every so often, or could that damage the stuff I want to grow?
Question: Do they need air to grow? There will be some (obviously), but should I open cabinet and box periodically to get some fresh air in, or would that be bad?
I know there may not be definite answers, but if anything is a "red flag" please let me know.
Thank you so much.