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Agar Medium sterilization

mmv

Neurotransmetteur
Inscrit
11 Mai 2007
Messages
42
I have 3 agar plates.. with solid medium. Do you think i can sterilize it in the pressure cooker? I've seen a movie where it says that you have to sterilize the agar whil it is LIQUID. but my agar is solid.. what do you think? Will the agar still be fine?
 

Roeligan

Glandeuse pinéale
Inscrit
22 Mai 2007
Messages
126
if nothing grows on the agar the day after 'solidification' it already is sterile.
I don't think the pressure cooker is a good idea, the agar would probably melt and/or caramellize
good luck growing anyway
 

mmv

Neurotransmetteur
Inscrit
11 Mai 2007
Messages
42
Indeed. the agar dishes melted :| I opened the pressure cooker, and instead of agar dishes, there were 3 plastic balls. :(
I'll get new dishes.. and won't sterilize them. Hope they will be already enough sterile.
 

Dantediv86

Holofractale de l'hypervérité
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18 Avr 2007
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2 264
wait you buy agar dishes allready made?
 

mmv

Neurotransmetteur
Inscrit
11 Mai 2007
Messages
42
umm no, actually, i 'get' them already made :D from a laboratory.. :p
i... know the right people :D
anyway. just got new ones. pus some drops of spores on them. now im waiting to see what's happening. 8)
 

Dantediv86

Holofractale de l'hypervérité
Inscrit
18 Avr 2007
Messages
2 264
goody lucky :D
 
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